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Antigens and Antibodies

Published May 2, 2012 6:26 AM by Scott Warner

The other day, a physician’s assistant wrote an order for “H pylori Ab” on a lab requisition. One of the techs telephoned: IgG, IgA, or IgM? Naturally, he ordered all three and hung up. And I thought, this is getting a bit out of hand.

Did we ask the wrong question? When do we cross the line in questioning antigens and antibodies?

Guidelines published in the American Journal of Gastroenterology state “The urea breath test and stool antigen test are currently the most accurate noninvasive diagnostic tools and can be used with confidence... Cost-effectiveness studies suggest that the stool test and the urea breath test that detect active infection are preferable to serological tests in the United States.”

Insurance carriers (e.g. Aetna and Anthem) have adopted American Gastroenterological Association and American College of Gastroenterology guidelines to eliminate serology testing for active infection.

ARUP laboratories has a helpful flowchart algorithm for H. pylori testing that doesn’t list antibody testing. If the patient is over age 55 or has “alarm symptoms,” an esophagogastroduodenoscopy (that’s a mouthful, ha ha) is done, otherwise an H. pylori breath test or stool antigen test is performed.

Finally, WebMD -- read by patients -- summarizes Helicobacter pylori testing: “If you have antibodies to H. pylori in your blood, it means you either are currently infected or have been infected in the past.” For at least ten years I’ve promoted the stool antigen test “to support a diagnosis of H. pylori infection or to determine whether treatment for an H. pylori infection has been successful.”

Should we steer providers to order tests? Instead of asking, “Which antibody test do you want, because our reference lab does three of them?” ask, “Are you looking for active infection?”

If the PA answers, “Yes,” we can say, “The guidelines for adults say to perform a urea breath test or stool antigen test instead,” and offer to fax the algorithm. It’s appropriate to add information about test sensitivity and even give an opinion.

Does your laboratory ask what kind of antibody, switch to antigen testing, or ask the reason for the test?

NEXT: Sustaining Change

7 comments

William,

Right you are!  Thanks.

Scott Warner May 11, 2012 6:25 AM

Amy,

I'll admit to not knowing when to order IgA, IgG, or IgM, at least regarding H. pylori testing.  (Maybe someone else can answer that.)  Initially, an IgG test was commonly ordered.  I think you're correct in assuming that some doctors and nurses aren't sure, either.

Generally, however: IgA is related to cellular / mucosal immunity and found in the digestive tract, nose, lungs, etc.; IgG is related to long-term immunity and is trans-placental; IgM is related to initial immunity.  An H. pylori IgG antibody test, for example, will tell you if a patient has been infected in the past.

Scott Warner May 11, 2012 6:21 AM

This article was talking about Ab's verus Ag's but I would like alittle clarity on the difference between IgA, IgG, and IgM? When to order which one because it is true some doctors and/or nurses are not sure what test they want so they do say "do all three". I always have to look it up somewhere to remember because I'm at a university and we do not do alot of this type testing. If at some point an article comes out please let me know. Thx Amy Edwards MLT

amy, general - MLT, UAP Clinic May 10, 2012 1:50 PM
terre haute IN

Great article, physicians are not god the are part of a professional team

Aaron Birch, generalist - MT, seattle va May 9, 2012 8:10 PM
Seattle WA

A small correction to the post, not directly related to the article.  The term physician's assistant is not correct, it should read "physician assistant"

William Plaster, MT, MLT (REt) May 8, 2012 6:22 PM
Summerville SC

Once again, it is the blur in the lines of can a Tech question an order, or just follow what the profider writes. I always advocate for the Technologists to be the frontline of assuring quality patient care! So this article is right on point, especially expressing the most effective way of communicating without causing the provider to question "Who are you to question my orders?"  It is our duty to protect the paitient, and to watchdog excessive or duplicate testing per regulations.  Don't be afraid to question.  Isn't that why we became Scientists?  Who, What, When, Why, Howcome.

John J. Havholm, Laboratory Consultant - DSc, MLS, CLS, CLT, Synergy Laboratories May 8, 2012 1:37 PM
Las Vegas NV

I enjoyed the article.  I viewed a webinar recently on lab utilization that ties in nicely with the material.  Thank you.

Lois, , Executive Coach Eagle Associates May 8, 2012 11:37 AM
MN

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    Scott Warner, MLT(ASCP)
    Occupation: Laboratory Manager
    Setting: Critical Access Hospital
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