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Spread the Word: A Medical Technology Student’s Perspective

Microbiology Rotation Part 1

Published July 10, 2009 8:29 AM by Tiffany Landis

On the first day of my microbiology rotation, I did not know what to expect. I got a tour of the hospital and a lesson on safety. I was then put at my first station, set ups, as this hospital calls it. I took worksheets with the patient information and tests ordered and found the specimen that matched it. I then plated the specimen to the appropriate media.

I also learned how Campylobacter grows in a 42 degree incubator with extra CO2. At the hospital it has its own special jar and incubator. Additionally, I learned how to screen for beta strep, gonorrhea, Clostridium difficile and learned how to make wet preps of yeast.

On the second day I was shown the QC that is performed every morning and how to change the gram stain solutions. I also discovered the joy of an automated gram stainer. I have been doing QC every morning now and I really got the hang of it. I really enjoyed setups. I learned which media to use--for instance, if I received a rectal swab and a lot of other things as well. So far I am really enjoying it. Next week I get to read plates on the stool and urine bench.

1 comments

Pseudomonas aeruginosa also grows at 42 C.  Our microbiology instructor at WFUBMC (Winston Salem, NC), who is now the MT program director there, used to make many different kinds of agar slant tubes and biochemical broth tubes (lysine, ornithine, arginine, etc.) as recently as 2004 when I attended the program because their clinical microbiology lab did a LOT of their identification procedures the "old-fashioned" way rather than using an automated system such as Vitek or WalkAway.

Another unique characteristic of P. aeruginosa is that its growth on a trypticase soy ("T-soy") agar slant glows when you put it under a Wood's lamp (a.k.a. "black light" or UV light).  I just read about several other cool diagnostic applications (in parasitic skin infections, etc.) of UV light on this page:

http://www.uvbnarrowband.com/?tag=woods-lamp

Other bacteria that you may not hear much about in MT school also like not only 42 C incubation temperatures, but extremely salty environments:

http://en.wikipedia.org/wiki/Halobacterium

I wrote a paper for one of my college microbiology courses about microorganisms who thrive in extremely COLD places.  In case you'd like to read more about these bacteria and fungi, here's another page I found:

http://www.daviddarling.info/encyclopedia/P/psychrophile.html

I think the hospital in Danville where I currently work used an automated Gram stainer in their microbiology lab before I came there, but they went back to using the bottled reagents and staining the "old-fashioned" way - probably because they saw as many overdecolorized Staphylococci as I did in WFUBMC's micro lab as a student when looking at slides that came off of THEIR Gram stain machine! LOL

Can you tell I love microbiology, by any chance? (I just need to wait for someone to retire off of day shift's Micro team in Danville, then perhaps I'll get to do more of it than loading blood cultures and seeing "NOS" on 99% of the CSF Gram stains I set up.)  

Stephanie Mathis, MT(ASCP), Generalist - Clinical Laboratory Scientist, Danville Regional Medical Center July 11, 2009 8:05 AM
Danville VA

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