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coverslipping and bubbles

Last post 02-10-2009, 8:31 PM by John Smith. 2 replies.
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  •  05-02-2005, 6:43 PM

    • englandl is not online. Last active: 11-27-2009, 6:50 PM englandl
    • Joined on 10-17-2007
    • Dir of Lab
    • Dreyer Med Clinic
    • Aurora , IL
    • 4 Posts

    coverslipping and bubbles

    Can anyone in the histology area help me solve the problem of bubbles developing under my coverslips?
  •  06-26-2007, 6:26 PM

    • Sulkosky is not online. Last active: 02-10-2009, 6:23 PM Sulkosky
    • Joined on 10-17-2007
    • Mgr Of Med Lab
    • Mon Gen Hosp
    • Morgantown , WV
    • 1 Posts

    RE:coverslipping and bubbles

    MESSAGE BY: englandl
    Can anyone in the histology area help me solve the problem of bubbles developing under my coverslips?
    REPLY FROM: SULKOSKY
    Are you performing automated or manual coverslipping? What kind of medium are you using?
  •  02-10-2009, 8:31 PM

    Re: RE:coverslipping and bubbles

    If you are using a mounting media such as cytoseal 60, it contains toluene which causes emulsions when mixed with water. Make sure you dehydrate thoroughly in 100% alcohol, and incorporate a clearing agent afterwards such as citri solv. try this. If it doesn't work immediately leave your slides to dry completely then try mounting. If you are somewhere really humid this can be tricky, you might have to turn up the air conditioning. Also, your mounting media may be contaminated, try using another labs or a fresh bottle if this solves the trick.